Journal: bioRxiv

Article Title: RodA Promotes Intestinal Colonization by Group B Streptococcus

doi: 10.1101/2025.10.27.684869

Figure Lengend Snippet: C. Adhesion assays were performed with Caco-2 and T84 intestinal cell lines respectively at an MOI of 1 to 10. B, D. Invasion assays were performed with Caco-2 and T84 intestinal cell lines respectively at an MOI of 1 to 10. The percentage of adhered and invasive bacteria were calculated based on the initial inoculum and expressed relative to the value for the WT strain. All data are from independent experiments (n=9, each with 3 technical replicates). The error bars indicate the 95% confidence intervals of the means of the wells. Data were analyzed using the Mann-Whitney U test (****, p < 0.0001) .

Article Snippet: The T84 colonic epithelial cell line (ATCC CCL-248) were cultivated in Dulbecco’s Modified Eagle’s Medium and Ham F-12 medium (DMEM: F-12, ATCC 30-2006) supplemented with 10% FBS and 1% Pen/Strep ( ).

Techniques: Bacteria, MANN-WHITNEY

Journal: Food Hydrocolloids for Health

Article Title: Pectins mitigate Candidalysin-induced gut barrier disruption and inflammation in vitro in a degree of methyl-esterification-dependent manner

doi: 10.1016/j.fhfh.2025.100232

Figure Lengend Snippet: Fig. 1. Treatment schedule and experimental design. T84 cells were seeded in ECIS plates until form a differentiated monolayer. Then, cells were pre-treated with 2 mg/mL of DM18 or DM88 pectin for 24 h (A). The next day, cells were exposed or to 10 µM CaLysin for 24 h (B). Untreated cells (complete medium with the vehicle) were used as negative control.

Article Snippet: To study the protective effects of lemon pectins against CaLysin barrier disruption and the expression of TJs-, inflammation- and wound healing-related genes, the human colorectal carcinoma epithelial cell line T84 (ATCC® CCL-248TM, Manassas, USA) was used in all the experiments.

Techniques: Negative Control

Journal: Food Hydrocolloids for Health

Article Title: Pectins mitigate Candidalysin-induced gut barrier disruption and inflammation in vitro in a degree of methyl-esterification-dependent manner

doi: 10.1016/j.fhfh.2025.100232

Figure Lengend Snippet: Fig. 2. The protective effect of DM18 and DM88 pectins on T84 epithelial intestinal barrier disruption induced by Candidalysin (CaLysin). Confluent monolayers of T84 cells were pre-incubated with 2 mg/mL of DM18 or DM88 for 24 h. Afterwards, the fungal toxin CaLysin (10 μM) was added, and the cells were incubated for 24 h. (A) The representative figure of barrier function measurement with ECIS system. (B) Calculated AUC. The % AUC of the T84 cells pre-incubated with only pectins were compared with the untreated controls. Statistical differences between the positive control and the different conditions were determined. Data from four independent experiments are presented as mean ± SEM. ** (p < 0.01) vs. Control or ## (p < 0.01) vs. CaLysin.

Article Snippet: To study the protective effects of lemon pectins against CaLysin barrier disruption and the expression of TJs-, inflammation- and wound healing-related genes, the human colorectal carcinoma epithelial cell line T84 (ATCC® CCL-248TM, Manassas, USA) was used in all the experiments.

Techniques: Disruption, Incubation, Positive Control, Control

Journal: Food Hydrocolloids for Health

Article Title: Pectins mitigate Candidalysin-induced gut barrier disruption and inflammation in vitro in a degree of methyl-esterification-dependent manner

doi: 10.1016/j.fhfh.2025.100232

Figure Lengend Snippet: Fig. 3. Pectin-mediated cytoprotection after CaLysin challenge. Determination of LDH (A) and ALDH (B) release in T84 cells expressed as Percent cytotoxicity. Data from four independent experiments are presented as mean ± SEM. * (p < 0.05), **** (p < 0.0001) vs. Control or # (p < 0.05), #### (p < 0.0001) vs. CaLysin.

Article Snippet: To study the protective effects of lemon pectins against CaLysin barrier disruption and the expression of TJs-, inflammation- and wound healing-related genes, the human colorectal carcinoma epithelial cell line T84 (ATCC® CCL-248TM, Manassas, USA) was used in all the experiments.

Techniques: Control